Kev siv cov tshuab lim membrane hauv kev tsim tshuaj tiv thaiv mRNA
Kev siv cov tshuab lim membrane hauv kev tsim tshuaj tiv thaiv mRNA
Kev taw qhia keeb kwm yav dhau
Tom qab ntau tshaj 30 xyoo ntawm kev tshawb fawb, cov tshuaj tiv thaiv mRNA tau txais kev saib xyuas zoo thaum lub sijhawm COVID-19 kis thoob ntiaj teb. Raws li cov cuab yeej siv tshuaj tiv thaiv tshiab, tshuaj tiv thaiv mRNA muaj qhov zoo ntawm kev tshawb fawb luv luv thiab kev loj hlob lub voj voog, tus nqi qis, ntau yam kev xaiv antigen thiab kev tiv thaiv zoo. Piv nrog rau cov tshuaj tiv thaiv subunit ib txwm muaj thiab cov tshuaj tiv thaiv tsis muaj zog, cov tshuaj tiv thaiv mRNA muaj peev xwm zoo thiab tau dhau los ua nees tsaus ntuj hauv thaj chaw tshuaj lom neeg, uas tuaj yeem ua rau muaj kev hloov pauv tsis zoo rau thaj chaw tshuaj lom neeg.
mRNA
Neeg xa xov liaison RNA (mRNA) yog ib hom ntawm ib leeg-stranded ribonucleic acid uas yog transcribed los ntawm ib tug strand ntawm DNA raws li ib tug qauv thiab nqa cov ntaub ntawv genetic ntaub ntawv uas yuav coj cov protein synthesis. Tom qab kev hloov pauv ntawm mRNA raws li lub hauv paus ntsiab lus ntawm kev sib koom ua ke raws li cov noob hauv cov hlwb, mRNA muaj cov kab ke sib txuas rau qee ntu ntawm DNA molecules, uas yog siv los ua tus qauv ncaj qha rau cov protein biosynthesis. Txawm hais tias mRNA tsuas yog suav txog 2% mus rau 5% ntawm tag nrho RNA ntawm lub xovtooj, nws yog qhov sib txawv tshaj plaws, thiab cov metabolism hauv lub cev muaj zog heev, thiab nws yog ib hom RNA nrog luv tshaj ib nrab-lub neej, uas decomposed nyob rau hauv ob peb. feeb mus rau ob peb teev tom qab synthesis.
tshuaj tiv thaiv mRNA
mRNA yog ib qho tshwm sim molecule uas nqa cov "blueprint" ntawm tib neeg lub hlwb los tsim cov hom phiaj proteins, los yog immunogens, uas ua kom lub cev tiv thaiv kab mob hauv lub cev los tua ntau yam kab mob. Cov tshuaj tiv thaiv mRNA siv cov caj ces ntawm tus kab mob es tsis yog tus kab mob nws tus kheej, yog li cov tshuaj tiv thaiv mRNA tsis muaj cov kab mob sib kis thiab tsis muaj kev pheej hmoo kis mob. Nyob rau tib lub sijhawm, cov tshuaj tiv thaiv mRNA tseem muaj kev tshawb fawb luv luv thiab kev loj hlob, uas tuaj yeem tsim cov tshuaj tiv thaiv tus neeg sib tw sai sai los daws cov kab mob sib txawv. Lub dual mechanism ntawm humoral thiab T cell tiv thaiv kab mob, muaj zog immunogenicity, tsis xav tau rau adjuvants, thiab yooj yim ntawm huab hwm coj ntau lawm txhawb lub ntsiab zoo ntawm lub ntiaj teb no mov. mRNA tshuaj tiv thaiv yog ib hom tshuaj tiv thaiv kab mob nucleic acid tshiab, uas yog cov tshuaj tiv thaiv tiam thib peb. Piv nrog rau cov tshuaj tiv thaiv ib txwm muaj, cov tshuaj tiv thaiv mRNA zoo ib yam li cov kab mob sib kis, thiab los ntawm kev txhaj tshuaj raws li kev qhia ntawm cov kws kho mob, lawv tuaj yeem tsim cov S protein hauv tib neeg lub cev, thiab tom qab ntawd txhawb lub cev los tsim cov tshuaj tiv thaiv kab mob los ntawm simulating kab mob. mRNA tshuaj tiv thaiv muaj qhov zoo ntawm kev qhia ceev ceev intracellular, cov lus teb ceev ceev, muaj zog tiv thaiv kab mob, kev ua tau zoo thiab kev nyab xeeb, kev tshawb fawb luv luv thiab kev loj hlob lub voj voog, thiab kev tsim khoom loj loj yooj yim. Hauv ob peb xyoos dhau los, ntau hom tshuaj tiv thaiv mRNA rau rabies, mob khaub thuas thiab lwm yam kab mob sib kis tau nkag mus rau hauv kev sim tshuaj thiab pom tias muaj kev cia siab rau daim ntawv thov. Thaum muaj tus kab mob COVID-19 kev sib kis, kev siv tshuaj tiv thaiv mRNA ua tiav tau txuas ntxiv siv lub platform thiab qhib lub qhov dej nyab rau kev siv cov tshuaj tiv thaiv mRNA hauv kev tiv thaiv kab mob sib kis, tshwj xeeb tshaj yog hauv cov kws kho tsiaj.
mRNA txheej txheem tsim tshuaj tiv thaiv
Qhov tshwm sim tam sim ntawm COVID-19 thaum kawg ntawm 2019 tau hloov pauv thaj chaw ntawm kev lag luam biomedical. mRNA tshuaj tiv thaiv thev naus laus zis tau tshawb nrhiav thiab tseem yog vim muaj kev siv zog txuas ntxiv los ntawm cov kws tshawb fawb hauv 30 xyoo dhau los, ua rau nws muaj peev xwm siv tau rau tib neeg. Kab mob yog cov kab mob sib txawv heev, thiab mRNA-based tshuaj tiv thaiv thev naus laus zis tuaj yeem hloov kho cov hom phiaj protein sai thiab ua tiav cov khoom lag luam sai thiab loj, uas muaj txiaj ntsig zoo rau kev tiv thaiv kab mob kis kab mob. COVID-19 tau qhib qhov rooj rau cov tshuaj tiv thaiv mRNA thiab mRNA tshuaj tiv thaiv txoj hauv kev rau lwm yam kab mob loj, thiab qhov cuam tshuam thiab qhov tseem ceeb ntawm kev txhawb nqa mRNA-raws li cov tshuaj tiv thaiv thev naus laus zis tuaj yeem txuas mus deb dhau ntawm COVID-19 tiv thaiv nws tus kheej.
Nyob rau hauv xyoo tas los no, cov tshuaj tiv thaiv mRNA tau ua tiav zoo, thiab kev siv thev naus laus zis hauv kev tiv thaiv thiab kho ntau hom qog thiab kis kab mob tau maj mam tsim thiab loj hlob, thiab ntau yam tshuaj tiv thaiv mRNA tau nkag mus rau kev sim tshuaj. Txawm li cas los xij, raws li kev siv tshuaj tiv thaiv tshiab, tshuaj tiv thaiv mRNA tseem ntsib qee qhov teeb meem thiab cov teeb meem hauv kev tshawb fawb thiab kev tsim kho, thiab tseem muaj ntau yam teeb meem uas yuav tsum tau kov yeej hauv txoj kev txhim kho. Yuav kom paub txog kev kho mRNA rau cov neeg mob tiag tiag, peb yuav tsum tau txheeb xyuas cov teeb meem tas li, nrhiav teeb meem, thiab daws teeb meem.
Tam sim no, cov txheej txheem tsim cov tshuaj tiv thaiv mRNA yog qhov nyuaj thiab tsis yooj yim, tab sis cov tshuab lim membrane khiav los ntawm tag nrho cov txheej txheem ntawm cov tshuaj tiv thaiv mRNA.
Membrane filtration txheej txheem
Membrane filtration technology yog hais txog cov txheej txheem sib cais membrane uas tau tsav los ntawm lub siab. Nyob rau hauv ib qho kev siab, thaum cov kua ntws los ntawm qhov chaw ntawm zaj duab xis, ntau qhov pores me me ntawm cov yeeb yaj kiab tsuas yog tso cai rau cov dej thiab cov molecules me me dhau mus thiab dhau los ua cov kua permeating, thiab cov khoom hauv cov kua uas nws ntim loj dua. tshaj li lub microaperture ntawm qhov chaw ntawm zaj duab xis yog daig nyob rau hauv cov kua inlet sab ntawm cov zaj duab xis, ua cov concentrated kua, thiaj li mus cuag lub hom phiaj ntawm kev sib cais thiab concentrating cov kua. Membrane filtration, raws li ib tug tshiab high-efficiency sib cais, concentration, purification thiab purification technology, muaj qhov zoo ntawm kev ua haujlwm yooj yim, me me hauv pem teb qhov chaw, tsis muaj theem hloov thiab tsis muaj kuab paug tshiab hauv cov txheej txheem kev kho mob, kev sib cais zoo, thiab lwm yam., muaj tsim sai heev nyob rau hauv lub dhau los 30 xyoo, thiab tau dav siv nyob rau hauv petrochemical, lub teeb kev lag luam textile, zaub mov, tshuaj, ib puag ncig tiv thaiv thiab lwm yam teb.
Kev siv cov tshuab lim membrane hauv kev tsim tshuaj tiv thaiv mRNA
Hauv ntau theem ntawm cov tshuaj tiv thaiv mRNA, sib txawv tangential flow filtration forms thiab membrane filtration products yuav tsum tau siv vim yog cov khoom sib txawv, impurities thiab cov hom phiaj kev sim.
(1) LNP ultrafiltration purification/concentration
Tom qab mRNA tau ua ke nrog cov khoom siv tau zoo (microfluidic ntim LNP), cov txheej txheem ultrafiltration purification / concentration yuav tsum tau ua. Nyob rau hauv tus txheej txheem ntawm mRNA tshuaj tiv thaiv, lub hnab ntawv membrane lossis hollow fiber nrog qhov tsim nyog aperture tuaj yeem raug xaiv raws li qhov loj me ntawm mRNA-LNP complex kom tshem tawm cov tsis sib xyaw mRNA thiab cov lipids dawb, ua kom cov concentration ntawm mRNA-LNP complex, hloov cov tshuaj tiv thaiv mRNA. tsis, kho tus nqi pH, thiab lwm yam.
(2) sterilization thiab filtration
Cov txheej txheem bactericidal filtration yog cov txheej txheem tsim khoom kom tau txais cov lim dej tsis huv los ntawm kev tshem cov kab mob hauv cov kua dej los ntawm cov kab mob bactericidal. Cov txheej txheem ntawm bactericidal filtration yuav tsum tsis txhob cuam tshuam rau qhov zoo ntawm cov khoom. Hauv cov txheej txheem tsim cov tshuaj tiv thaiv mRNA, lub lim uas muaj qhov pore loj ntawm 0.22μm feem ntau yog siv los tshem tawm cov kab mob microbial xws li kab mob thiab txhim kho kev nyab xeeb ntawm cov tshuaj tiv thaiv mRNA.
Hais txog Guidling
Guidling Technology yog lub teb chaws high-tech enterprise tsom mus rau biopharmaceuticals, cell culture, purification thiab concentration ntawm biomedicine, kuaj mob thiab muaj kua. Peb tau ua tiav cov khoom siv lim dej centrifugal, ultrafiltration & microfiltration cassettes, cov kab mob lim, TFF system, qhov tob lim, hollow fiber, thiab lwm yam. Uas tau raws li daim ntawv thov scenarios ntawm biopharmaceuticals, kab lis kev cai ntawm tes, thiab lwm yam. Peb cov membranes thiab cov lim dej lim tau dav siv hauv kev sib xyaw, kev rho tawm thiab kev sib cais ntawm kev pom ua ntej, microfiltration, ultrafiltration thiab nanofiltration. Peb cov kab ntau cov khoom, los ntawm me me, ib leeg-siv chav kuaj pom mus rau ntau lawm filtration systems, sterility kuaj, fermentation, cell kab lis kev cai thiab lwm yam, ua tau raws li cov kev xav tau ntawm kev sim thiab ntau lawm. Guidling Technology tab tom tos ntsoov rau kev koom tes nrog koj!
