Zam kev ua tsis tiav! Kho cov tswv yim kom txo qis rau shear kev ntxhov siab hauv lub tshuab hollow
Zam kev begopoduct inactivation!
Ua kom meej cov tswv yim xam thiab txo cov tswv yim rau shear kev ntxhov siab hauv hollow fiber systems
Hollow Fiberyog hom fiber ntau nrog lub hollow sab hauv cov channel thiab ntxig lossis tuab polymer membrane thaum nws sab nraud phab ntsa sab nraud. Tus qauv tshwj xeeb no muaj lub thaj chaw siab, kev hloov pauv loj heev, thiab muaj zog txhua yam muaj zog. Tsav los ntawm tangers siab, hollow cov kab ke lim thiab cov kab mob lossis rov qab cov tshuaj tiv thaiv los ntawm kev xaiv portability. Lawv tau siv dav hauv teb xws li biomedicine, bioengineering, thiab kev tiv thaiv ib puag ncig.
Cov khoom lag luam zoo
Qhib Flow raws nrog siab particle -} tuav lub peev xwm
Zoo nkauj tshiab tso cai nrog ua tiav ntau qhov ntau thiab tsawg
Saj zawg zog cov qauv tsim rau txoj kab yooj yim - Up
Tsawg shear quab yuam, zoo tagnrho rau kev tuav cov tshuaj tiv thaiv zoo thiab cov khoom lag luam
Shear kev ntxhov siab hauvHollow Fiber SystemsUa si lub luag haujlwm tseem ceeb hauv kev tsim khoom, kev siv tshuaj tiv thaiv ntawm cov tshuaj tiv thaiv monopronte, tshuaj tiv thaiv kab mob monoclonal, tshuaj tiv thaiv cov tshuaj, thiab kho mob cell. Thaum lub sij hawm tsav dav hlau hnyav tuaj yeem txhim kho kev hloov pauv loj thiab sib xyaw, ntau dhau shear tuaj yeem ua rau cov khoom lag luam tsis sib haum, sib sau ua ke, lossis txawm tias kev puas tsuaj ntawm tes.
Tus tub ntxhais tseem ceeb ntawm hollow cov lus dag nyob hauv lawvQhib - channel qauvCov. Tsis zoo li flag - ntawv} ntawv cassettes nrog cov kua hauv cov dej sab hauv muaj cov ntaub ntawv sab hauv muaj zog, hollow cov hmoov av loj tsim los ntawmTxaus tus nqithiabFiber NtauCov. Qhov no yooj yim tso cai rau kev tswj cov rog los ntawm kev kho ob qho chaw maj rau shear- cov qauv ntsuas ntawm lawv cov qauv kev ntsuas.
Tusshear tus nqi ()Nyob rau hauv ib hollow fiber tuaj yeem suav los ntawm cov kab sib luag hauv qab no:
=} shear tus nqi (s⁻¹)
Q= Tangent nrog tus nqi ntws
n=} tus lej ntawm fibers
r=} sab hauv hluav taws xob ntawm txhua qhov fiber ntau
Cov mis no qhia tau tias Shear tus nqi yogncaj qha sib ncau ncaj qha rau kev ntws ntwsthiabinversely proportional rau tus naj npawb ntawm fibersThiab tuslub voos xwm txheej ntawm cov ntsuas hluav taws xobCov. Hauv kev xyaum, shear kev nyuaj siab tuaj yeem tswj tau los ntawm kev hloov kho tus nqi ntws, cov kev teeb tsa kev teeb tsa ntau lossis cov qauv kev tsim kho kom tau raws cov txheej txheem tshwj xeeb.
Kev cuam tshuam ncaj qha ntawm shear kev nyuaj siab ntawm bioproducts
Protein / Antiby Dissaturation thiab kev sib sau ua ke
Siab shear rog - Xws li kev kub lossis cavitation - tuaj yeem ntxiasKev Hloov Pauv HloovHauv cov protein, cov cheeb tsam hydrophobic thiab ua rau muaj kev sib sau ua ke. Lub sijhawm lav, ultrefiltration, lossis perfusion kab lis kev cai, ntau dhau ntawm kev ntxim nyiam ntxim nyiam yuav cuam tshuam cov qauv protein cov qauv.
Piv txwv li:Monoclonal tshuaj tiv thaiv (MABS) nyiam ua kev sib sau ua ke thaum lub siab - ceev prictration lossis membrane pom, cuam tshuam rau ob qho tib siKev ua tau zoo thiab kev nyab xeeb.
(2) kev puas hlwb (cov tsiaj thiab cov microbial)
MOBMALIAN HLI (piv txwv li, Cho cells) yog shear - rhiab. Ntau dhau shear tuaj yeem ua raumembrane rupture, kev npau liab, lossismetabolic tsis sib haum.
Cov kab mob me me (piv txwv li,E. coli) tej zaum yuav yiag yauvLysis CellNyob rau hauv lub siab shear, tso cov osotoxins.
Qhov ntsuas tseem ceeb:
Cov tsiaj muaj plaub hau: feem ntau yog ua kom tau<50–100 dyn/cm²(Hauv Perfusion Culture)
Cov ntshav liab: cov nqi shear>1500 s⁻¹tuaj yeem ua rau hemolysis (piv txwv li, hauv hemodialys)
(3) Puas tsuaj rau cov kab mob, exosomes, thiab lwm nanoparticles
Vital vectors (xws li Aav thiab Lentivirus) lossis exosomes tuaj yeem tawg nyob rau hauv cov kev ntxhov siab shear, ua rauTxo kev kis tauthiabTsawg dua kev kho mob.
Piv txwv li:Hauv kev kho cov cuab yeej siv, tswj cov kev nyuaj siab shear thaum Hollow Fiber Purification yog qhov tseem ceeb kom tiv thaiv tus kab mob poob.
(4) Membrane fouling thiab khoom poob
Siab shear yuav ua rauCov khib nyiab ntawm tes lossis cov proteinTxhawm rau khaws ntawm daim nyias nyias, thaiv cov pores thiab txo cov neeg hloov chaw loj.
Shear -} induced adsorption - xws liCov Tshuaj Tiv Thaiv Nonspecious Tshuaj Tiv ThaivMus rau membrane Surfaces - tuaj yeem qis dua cov khoom rov qab.
Cov tswv yim los txo cov kev cuam tshuam shear kev ntxhov siab
(1) Tsim Txheej Txheem Kev Tsim Kho Kom Zoo
Txo kev ntws nrawm:Siv qis - shear twj (piv txwv li, cov twj tso kua mis (piv txwv li optimized opved kab geometries (piv txwv li, taper raws).
Xaiv kev xaiv Fiber:Nce Fiber hauv Fiber hauv Fiber hauv Fiber hauv cov phab ntsa fiber ntau kom txo phab ntsa shear kev ntxhov siab (sib npaug ntawm huab hwm coj hloov efficiency).
Kho qauv:Siv cov txheej hydrophilic kom txo qis protein adsorption.
(2) txheej txheem ntsuas hluav taws xob
Ncau kab lis kev cai:Saib xyuas cov nqi tsawg dua (piv txwv li, 1-3 RV / hnub) kom tsis txhob muaj mob ntawm tes puas.
Hloov chaw tangential Flow (ATF):Siv cov sib quas ntus ntws los txo cov siab ua kom tiav siab.
Purification:Thaum muaj ultraafiltration / Diafiltration, siv cov transmembrane siab (Tmp<1 bar) thiab txo tus nqi txaus.
(3) Cov tshuaj ntxiv tiv thaiv thiab cov txhab ruaj khov
Protein Stabilizers:Ntxiv cov suab thaj (piv txwv li, trehalpe) lossis surfactants (piv txwv li, pluronic F68) kom txo qis kev sib cais.
Kev tiv thaiv ntawm tes:Ntxiv cov ntshav dej lossis cov polymers (piv txwv li, kev haus dej cawv polyvinyl) txhawm rau txhim kho cov phom sij shear.
(4) Kev Ntsuas Online Online thiab Ua Qauv
Sensors:Kev ua haujlwm phab ntsa shear nyuaj siab sensors rau tiag - lub sijhawm tshuaj xyuas.
CFD qauv:Siv cov kua dej ua ke (CFD) los kwv yees siab siab siab {{
Qhov Zoo ntawm Hollow Fiber Systems hauv Biroproduc
Hollow Fiber Systemine Lub TshuabTsawg - shear tsimuas muaj cov txiaj ntsig zoo rau kev uashear -} Cov ntaub ntawv rhiabxws li cov protein, vectors vectors, thiab hlwb.
Lawv litangential khiav pom kev (TFF)Kev teeb tsa minimizes transmembrane siab (tmp) los ntawm parallel ntws, txo cov kua shear thiab tiv thaiv cov khoom lag luam denaturation lossis kev puas tsuaj.
Tusmodular tsimtxhawb nqaLINEAR Nplai -}, kom ua kom nruj sib xws shear tsis los ntawm ib sim mus rau ntau lawm thiab tswj khoom ruaj ntseg. Tsis tas li ntawd, kev sib xyaw ua keHydrophilic Membrane Khoom(piv txwv li, pes, PVDF) nrogTsawg - shear twj(xws li, diaphragm twj) minimizes kev sib txhuam thiab kev tshaj tawm, ua tiav kev rov ua tiav siab- Piv txwv,AAV recovery rates >90%.
Hauv cov ntsiab lus, Hollow fiber ntau systems, nrog lawvTsawg shear kev ntxhov siab, Kev Tsim Nyog, thiabkev loj, sawv cev ib qho kev daws teeb meem hauv qabhigh - tus nqi thiab shear -}} rhiab biropoducts.
