Thaum Xaiv Lub Tangential Flow Filtration (TFF) System, Peb Puas Yuav Siv Lub Hollow Fiber Cartridge lossis Ib Daim Ntawv Cassette Membrane?
Tangential Flow Filtration (TFF) yog dav siv nyob rau hauv ob qho tib si biopharmaceutical thiab tshuaj kws tshuaj raws li ib tug tseem ceeb technology rau buffer pauv thiab purification. Ntawm tag nrho cov txheej txheem cov txheej txheem, kev xaiv ntawm cov membrane cassette los yog hollow fiber module yog qhov tseem ceeb tshwj xeeb
Ua ntej, qhia luv luv rau hollow fiber modules thiab membrane cassettes:
Hollow Fiber Module:
Cov tshuaj txau ntws axially los ntawm lub lumen ntawm lub hollow fibers, thaum lub permeate dhau radially los ntawm cov phab ntsa membrane.
Membrane Cassette:
Ntau lub tiaj tus- daim ntawv daim nyias nyias tau muab tso ua ke, feem ntau sib cais los ntawm cov ntxaij vab tshaus los yog ntws spacers uas tsim tangential ntws raws los txhim kho huab hwm coj hloov.
1. Kev sib piv ntawm Cov Kev Ua Haujlwm Tseem Ceeb
A. Flux / Mass Transfer Efficiency (Kev Ua Haujlwm Ceev)
Membrane cassettes feem ntau ua tiav cov flux ntau dua thiab siv sijhawm luv dua.
Lub spacer / screen qauv tsim turbulence ntawm daim nyias nyias, txo cov gel txheej tsim thiab txhim kho huab hwm coj hloov efficiency thiab tag nrho cov flux kev ua tau zoo.
Hollow fiber modules feem ntau muab qis dua tab sis ruaj khov dua.
Lawv txoj kev qhib ntws txo qis hydraulic tsis kam, ua rau lawv tsis tshua muaj kev cuam tshuam sai sai thaum ua cov khoom siab - khoom lossis siab - viscosity pub kwj.
Piv txwv li:
Hauv kev ua UF/DF ntawm LNP-mRNA, cov kev tshawb fawb tau pom tias nyob rau hauv cov xwm txheej TMP zoo tib yam (tsawg dua lossis sib npaug li 5 psi) thiab cov khoom noj tsis zoo, cov khoom siv hauv daim nyias nyias tau pom tias muaj ntau dua flux, thaum hollow fiber modules muaj qis shear tab sis txo qis me ntsis.
B. Shear Force (Kev cuam tshuam rau cov khoom, kab mob, thiab biomolecules)
Hollow fiber modules feem ntau suav hais tias yog softer.
Lawv qhib-channel tsim txhais tau hais tias shear feem ntau ua raws li tus qauv tubular ntws tus cwj pwm thiab tuaj yeem tswj tau ntau npaum li cas los ntawm tus nqi ntws thiab fiber ntau sab hauv txoj kab uas hla, ua rau lawv haum rau shear- cov ntaub ntawv rhiab heev.
Membrane cassettes qhia ntxiv shear vim lub spacer qauv.
Txawm hais tias qhov no txhim kho kev hloov pauv huab cua, nws kuj tseem tuaj yeem ua rau muaj kev ntxhov siab hauv zos.
C. Fouling Resistance / tuav cov khoom siab thiab siab viscosity
Hollow fiber modules qhia pom qhov zoo dua thaum ua cov khoom siab -khoom- cov ntsiab lus lossis cov khoom khov.
Txoj kev qhib ntws txo qis cov dej tsis kam thiab pab tswj cov flux ruaj khov raws li qhov xav tau ntawm cov khoom noj.
Membrane cassettes feem ntau yog tsim nyog rau qis - mus rau - cov ntaub ntawv siv cov khoom nruab nrab uas muaj kev nkag siab thiab kev ua haujlwm sai dua yog qhov tseem ceeb.
Lawv txhim kho kev hloov pauv loj tuaj yeem xa cov kev ua tau zoo, txawm hais tias khov heev lossis siab -cov khoom siv yuav muaj kev poob qis dua nyob ntawm cov khoom siv membrane, MWCO / pore loj, thiab cov txheej txheem txheej txheem.
D. Siab poob thiab lub qhov rais ua haujlwm (TMP & Pub Siab)
Hauv hollow fiber systems, siab poob thiab shear tuaj yeem tswj tau los ntawm fiber ntau txoj kab uas hla, fiber ntau ntev, thiab crossflow tus nqi.
Cov kab me me fiber ntau nce shear, thaum txoj kev khiav ntev dua nce siab poob.
Hauv kev xyaum, hollow fiber TFF systems feem ntau tsim thiab scaled siv TMP-raws li kev xam.
E. Concentration Factor, Recovery Rate, thiab Tuav-Up Volume
Membrane cassettes feem ntau muaj txiaj ntsig zoo rau siab - cov ntawv thov kev xav thiab cov ntsiab lus siab.
Kev lag luam kev paub qhia tias daim nyias nyias nyias muaj cov qauv sib txuam, kev ua haujlwm siab zoo dua, muaj peev xwm muaj peev xwm ntau dua, thiab feem ntau cov khoom rov qab zoo dua piv nrog cov tshuab fiber ntau.
Hollow fiber modules yuav muaj qhov tuav loj dua - nce ntim nyob ntawm cov qauv tsim thiab kev teeb tsa, uas tuaj yeem ua qhov tseem ceeb dua thaum ua cov khoom me me ntawm cov khoom muaj txiaj ntsig.
F. Kev Ntseeg thiab Kev Pabcuam Lub Neej: Fiber Breakage vs. Maintenance
Ib qho kev pheej hmoo ntawm hollow fiber systems yog fiber ntau breakage thiab cov qauv fragility.
Cov kev paub dhau los tau pom tias cov fibers hollow tuaj yeem tawg qee zaus, thiab qee qhov pore cov qauv (xws li ntiv tes-zoo li cov qauv) yuav raug puas tsuaj ntau dua.
Membrane cassettes kuj xav tau kev saib xyuas kom zoo, tab sis lawv lub neej kev pabcuam tuaj yeem ntev heev nrog kev tu thiab khaws cia cov txheej txheem tsim nyog.
Nws yog ib qho tseem ceeb kom tsis txhob ziab tawm ntawm daim nyias nyias thiab rov tsim dua daim nyias nyias tam sim tom qab fouling.
G. Ib leeg-Siv, Sterility, thiab Scale-Up
Ib leeg-siv hollow fiber modules tau nrov zuj zus vim tias lawv feem ntau muab cov khoom ua ntej-wetted, gamma sterilized, thiab npaj siv, txo cov kev tu kom huv si.
Lawv kuj tuaj yeem ntsuas tau zoo los ntawm kev tsim kho hauv chav kuaj mus rau tag nrho - nplai tsim.
Scale- nce logic:
Ob leeg hollow fiber modules thiab membrane cassettes tuaj yeem scaled los ntawm kev nce daim nyias nyias cheeb tsam los yog ua hauj lwm ntau units nyob rau hauv parallel. Hollow fiber ntau cov ntaub ntawv tseem hais ntxiv tias muaj ntau qhov ntau thiab tsawg, ua kom ncaj ncaj scale -nce ntawm qhov chaw kuaj mus rau qhov ntau lawm.
2. Cov lus sib piv xaiv
Dimension Hollow fiber ntau cassettes
