Txoj Cai Thiab Daim Ntawv Thov Ntawm Tangential Flow Filtration Technology
Tangential flow filtration yog ib txoj hauv kev zoo rau kev tsom thiab tshem tawm 10ml mus rau ntau txhiab litres ntawm cov qauv tshuaj. Nws tuaj yeem siv los cais cov biomolecules loj los ntawm me me biomolecules, ntes cell suspensions, thiab qhia meej fermentation broth thiab cell lysates. TFF tuaj yeem siv rau ntau yam kev siv, suav nrog cov tshuaj lom neeg protein, molecular biology, immunology, biochemistry thiab microbiology. Daim ntawv no yuav tsom mus rau lub hauv paus ntsiab lus, qhov tsis tseem ceeb thiab kev siv tangential flow filtration technology.
Yuav ua li cas xaiv qhov tsim nyog tangential flow filtration system
TFF yog tangential flow filtration, uas yog lub zog tsav cov txheej txheem sib cais raws li molecular loj. Nrog TFF, cov qauv sib xyaw tsis raug yuam los ntawm ib txoj hauv kev kom dhau los ntawm daim nyias nyias zoo li DC pom. Hloov chaw, cov kua dej ntws tawm tangentially los ntawm qhov chaw ntawm daim nyias nyias los ntawm ntau zaus. Qhov "sweeping" tus cwj pwm no tshwm sim los ntawm kev siv lub siab txo qhov tsub zuj zuj ntawm thawj cov qauv ntawm daim nyias nyias. Lub hom phiaj molecules nrog qhov hnyav molecular dua li qhov cuam tshuam los ntawm daim nyias nyias tau khaws cia, tab sis cov khoom me me thiab tsis tau dhau los ntawm daim nyias nyias. Tangential flow filtration yog ib txoj hauv kev zoo rau kev tsom thiab tshem tawm 10ml mus rau ntau txhiab litres ntawm cov qauv tshuaj. Nws tuaj yeem siv los cais cov biomolecules loj los ntawm me me biomolecules, ntes cell suspensions, thiab qhia meej fermentation broth thiab cell lysates. TFF tuaj yeem siv rau ntau yam kev siv, suav nrog cov tshuaj lom neeg protein, molecular biology, immunology, biochemistry thiab microbiology. Daim ntawv no yuav tsom mus rau lub hauv paus ntsiab lus, qhov tsis tseem ceeb thiab kev siv tangential flow filtration technology.
Pa filtration txhais tau hais tias nyob rau hauv qhov kev txiav txim ntawm lub siab, cov kua ncaj qha mus rau hauv lub lim membrane thiab nkag mus rau hauv downstream, thaum loj hais los yog molecules yog intercepted upstream los yog nyob rau hauv daim nyias nyias, thiab me me hais los yog molecules nkag mus rau hauv downstream los ntawm daim nyias nyias. Nyob rau hauv hom kev ua haujlwm no, cov kev taw qhia ntws ntawm cov kua yog perpendicular mus rau daim nyias nyias thiab nkag mus rau hauv qab, yog li nws tseem hu ua "tuag kawg pom". Daim ntawv thov kev pom zoo suav nrog kev pom tseeb, kev ua kom tsis muaj menyuam thiab kev tshem tawm cov kab mob, uas tsis yog qhov tseem ceeb ntawm daim ntawv no. Tangential flow filtration txhais tau hais tias cov kev taw qhia ntws ntawm cov kua yog thaum uas tig mus rau daim nyias nyias. Nyob rau hauv qhov kev txiav txim siab, tsuas yog ib feem ntawm cov kua dhau los ntawm daim nyias nyias thiab nkag mus rau hauv qab. Hom kev ua haujlwm no tseem hu ua "cross flow filtration". Vim hais tias cov tangential ntws tsis tu ncua "ntxuav" saum npoo ntawm lub pob membrane thaum lub sij hawm pom cov txheej txheem, hom kev ua haujlwm no tuaj yeem txo qhov tsub zuj zuj ntawm cov khoom loj thiab cov molecules ntawm daim nyias nyias, uas ua rau hom kev ua haujlwm no muaj qhov tshwj xeeb zoo hauv ntau daim ntawv thov.
Hauv tangential flow (tseem hu ua "cross flow") filtration, lub twj tso kua mis thawb cov kua los ntawm qhov chaw ntawm lub lim membrane kom ntxuav tawm cov twj tso kua mis, thiaj li txo qis qhov ntsuas ntawm lub lim membrane. Nyob rau tib lub sijhawm, cov kua dej tangential tseem yuav ua kom lub siab perpendicular mus rau lub lim membrane, thawb cov kuab tshuaj thiab cov khoom me me los ntawm cov lim membrane. Ua li no, kev lim dej tuaj yeem ua tiav. Kev simulation ntawm kev sib cais cov xuab zeb thiab pebbles los ntawm subdivision screen yog pab kom nkag siab txog cov txheej txheem ntawm tangential flow filtration: lub qhov screen cim lub qhov hws ntawm lub lim membrane, thaum xuab zeb thiab pebbles cim lub molecules yuav tsum tau sib cais. Nyob rau hauv DC pom, cov xuab zeb pebble sib tov yog yuam kom txav mus rau ntawm lub qhov screen, thiab ib txheej pebble yog tsim rau ntawm qhov screen vim qee cov xuab zeb me me poob los ntawm lub qhov screen, tiv thaiv cov xuab zeb sab saum toj los ntawm kev txav mus los ntawm qhov screen thiab dhau mus. qhov screen mesh (Daim duab 1). Nyob rau hauv DC filtration, nce lub siab tuaj yeem tsuas yog siv lub zog ntawm qhov sib tov, uas tsis tsim nyog rau kev txhawb nqa kev sib cais; Nyob rau hauv sib piv, nyob rau hauv lub tangential flow filtration hom, tsim ntawm lub limiting txheej yog tiv thaiv los ntawm recirculation ntawm cov sib tov, uas yog zoo li kev co kom tshem tawm cov pebbles thaiv lub screen mesh, thiaj li hais tias cov xuab zeb nyob rau sab saum toj ntawm cov sib tov ntog thiab dhau ntawm qhov screen mesh. Yog li ntawd, kev siv tangential flow filtration rau biomolecular sib cais muaj ntau dua efficiency thiab ceev concentration los yog pom ceev.
