Cov lus teb rau Cov Lus Nug Cov Lus Nug Ntawm Ultrafiltration Centrifugal Pipe

1. Dab tsi yog cov khoom siv membrane ntawm lub raj ultrafiltration?

Polyethersulfone daim nyias nyias muaj qhov tseeb thiab meej qhov hnyav molecular cuam tshuam, ruaj khov lub cev thiab tshuaj lom neeg, tsis tshua muaj protein ntau adsorption thiab cov khoom siab rov qab.

2. Yog tias cov proteins uas muaj qhov hnyav molecular sib txawv yuav tsum tau sib cais, qhov sib txawv ntawm qhov hnyav molecular ntawm cov protein xav tau ntau npaum li cas?

Ob qho proteins yuav tsum yog 10 npaug lossis ntau dua qhov sib txawv

3. Ultrafiltration centrifuge raj puas siv tau rau cov kab mob concentration?

Ultrafiltration centrifuge raj tuaj yeem mloog cov kab mob ntau zaus lossis txawm tias lub sijhawm siab. Peb muaj daim nyias nyias tshwj xeeb rau tus kab mob. Cov kab mob sib txawv yuav tsum xaiv qhov tsim nyog daim nyias nyias pore loj

4. Ultrafiltration centrifuge raj puas tuaj yeem tua kab mob thiab alkali tiv taus?

Nws tuaj yeem muab tshuaj tua kab mob nrog 75 feem pua ​​​​ethanol aqueous tov. Peb cov khoom tuaj yeem tiv taus 0.1-0.5n sodium hydroxide alkaline ntxuav

5. Ultrafiltration centrifuge raj tuaj yeem siv rau kev ua kom tsis muaj zog siab

Peb muaj tshwj xeeb ultrafiltration centrifuge raj haum rau high-pressure sterilization

6. Xav txog kev ruaj ntseg ntawm cov protein, puas tuaj yeem ua lub raj ultrafiltration centrifuged ntawm qhov kub thiab txias?

Yog lawm, peb cov khoom yog resistant rau kub tsawg, tab sis lub sij hawm concentration yuav ntev dua. Qhov pom ceev ntawm daim nyias nyias muaj feem xyuam nrog cov kua dej pub

7. Hais txog tus nqi rov qab

Feem ntau, tus nqi rov qab los ntawm cov kua nplaum uas muaj ntau dua 0.1mg/ml yog 90 feem pua ​​lossis ntau dua. Feem ntau, qhov poob ntawm cov qauv yog tshwm sim los ntawm nonspecific adsorption ntawm qhov chaw ntawm lub lim membrane thiab qhov chaw ntawm cov khoom siv yeeb nkab. Txhawm rau kom tau txais kev rov zoo siab, qhov hnyav ntawm cov khoom siv lim dej uas tau xaiv yog kwv yees li 1/3 ntawm lub hom phiaj cov protein, ultrafiltration membrane yog asymmetric membrane, nws qhov pore loj yog ib txwm faib, thiab nws yuav xau hauv qab centrifugal siab. siab. Nyob rau hauv cov xwm txheej no, cov kua protein yuav deformed, yog li ntawd, qhov me me ntawm kev cuam tshuam aperture yog, qhov qis qis qis dua, tab sis tus nqi rov qab yuav siab dua.

8. Thaum cov protein yog concentrated, muaj nag lossis daus. Yuav ua li cas nrog nws?

Yog hais tias cov protein ntau ntau ceev los yog ntau dhau, nws yuav ua rau cov protein ntau los nag. Qhov kawg concentration tom qab protein concentration yuav tsum tsis txhob siab dhau; Rau cov proteins uas yog rhiab heev rau concentration npaum li cas thiab yooj yim rau precipitate, cov tswv yim kev txhim kho yog raws li nram no:

1) Txo qhov ceev centrifugal,

2) Siv ultrafiltration membrane nrog qhov hnyav dua molecular

3) Tshuab thiab nqus cov yeeb nkab concentration thiab ces centrifuge

9. Yuav ua li cas yog vim li cas vim li cas lub ultrafiltration centrifuge raj tsis tuaj yeem centrifuged cia?

Yog tias lub raj ultrafiltration tsis tuaj yeem centrifuged, thawj zaug centrifugation tuaj yeem ua tiav nrog txog 20 feem pua ​​​​ethanol aqueous tov, vim tias tom qab lub ultrafiltration membrane tau qhuav tag, daim nyias nyias yog tsis muaj zog hydrophobic, thiab cov flux tuaj yeem rov qab los tom qab soaking nrog qis nto. hnyav hnyav

10. Kev siv thiab khaws cia ntawm ultrafiltration raj

Lub raj ultrafiltration yuav tsum tau pib centrifuged nrog dej huv ua ntej siv los xyuas nws txoj kev ncaj ncees. Tom qab txhua qhov kev siv, nws yuav tsum tau khaws cia rau hauv lub xeev ntub dej kom tsis txhob muaj cov membrane kom qhuav

11. Yuav ua li cas tshem tawm endotoxin los ntawm ultrafiltration raj?

Lub raj ultrafiltration muab los ntawm peb tsis tau kho nrog endotoxin. Nyob rau tib lub sijhawm, vim muaj ntau yam endotoxins nyob rau hauv cov xwm txheej, cov qauv uas xav tau cov co toxins tuaj yeem raug ntxuav ua ntej nrog 0.5N sodium hydroxide ua ntej kev sim, thiab tom qab ntawd ntxuav nrog RO dej kom tshem tawm feem ntau ntawm cov endotoxin.

12. Thaum cov concentrated protein raug soj ntsuam hauv qab dej, nws pom tias muaj kev cuam tshuam. Yog vim li cas?

Lub raj ultrafiltration tuaj yeem ntxuav ua ntej nrog tsis muaj dej lossis dej huv. Yog tias tseem muaj kev cuam tshuam, ntxuav nws nrog 0.1N sodium hydroxide, thiab tom qab ntawd ntxuav nws nrog dej huv

13. Tej zaum yuav muaj nonspecific adsorption ntawm lub hom phiaj protein thiab ultrafiltration membrane nyob rau hauv cov txheej txheem concentration. Yuav ua li cas txhim kho nws?

Peb siv hydrophilic polyethersulfone membrane, tab sis rau qee cov proteins uas muaj cov khoom tshwj xeeb, lawv cov adsorption nonspecific nrog daim nyias nyias tuaj yeem txhim kho. Hauv qhov no, peb tuaj yeem sim pretreat lub raj ultrafiltration ua ntej qhov kev sim, lossis tiv tauj peb tus engineer

14. Tom qab cov concentration, nws pom tias tsis muaj lub hom phiaj protein nyob rau hauv cov concentrate. Yuav ua li cas yog vim li cas?

1) Thawj qhov concentration ntawm cov protein yuav tsum tsis txhob qis dhau. Yog tias qhov concentration tsawg dhau, lub membrane yuav tsis khaws cia

2) Xaiv qhov tsim nyog membrane pore loj. Centrifugation thiab concentration ntawm ultrafiltration raj belongs rau tuag kawg pom. Lub membrane pore loj yog feem ntau tsawg dua 2 zaug lossis ntau dua ntawm cov hom phiaj protein

3) Xaiv qhov tsim nyog centrifugal ceev

4) Seb lub hom phiaj protein precipitates