5ml Centrifugal Devices

1. Kev piav qhia
Guidling 5ml centrifugal li muab ceev ultrafiltration, nrog lub peev xwm rau siab concentration yam thiab yooj yim concentrate rov qab los ntawm dilute thiab complex qauv matrices. Qhov chaw muaj daim nyias nyias muab cov qauv ua qauv sai, cov qauv zoo rov qab (xws li feem ntau ntau dua 90 feem pua ​​​​ntawm cov tshuaj pib dilute) Lub sijhawm ua haujlwm yog 15 txog 25 feeb. Solute polarization thiab tom qab fouling ntawm daim nyias nyias yog qhov tsawg kawg nkaus los ntawm kev tsim ntsug, thiab lub cev tuag nres hauv cov cuab yeej lim tiv thaiv kom tsis txhob tig mus rau qhov ziab thiab qhov ua piv txwv poob. Lub ultrafiltrate yog sau nyob rau hauv lub muab centrifuge raj. Lub 5ml centrifugal li tuaj yeem spun rau hauv lub thoob viav vias (rau kev ua haujlwm zoo) lossis lub kaum sab xis rotor. Guidling 5ml centrifugal li yog muab tsis-sterile thiab yog siv rau ib leeg xwb

2.Specifications
Cov khoom siv ntawm kev tsim kho
Cov khoom siv xaiv los ntawm ntim: 0.5ml, 5ml, 15ml
Lim Media: polyethersulfone membranes
Sample Reservoir, Filtrate Receiver thiab Cap: Polypropylene
Paddle: polycarbonate
pH ntau: 1 - 14
Maximum Centrifugal Force
7,500 xg (Ultrafiltration)
Kev huv huv
Muab tsis-sterile. Tej zaum yuav ua kom huv los ntawm kev lim dej 60-75 feem pua ​​​​ethanol los ntawm lub cuab yeej ua ntej siv .
Guidling centrifugal lim ntaus ntawv yog muab nrog lub hau, lub tshuab lim, thiab lub raj centrifuge.

Cov Khoom Siv Yuav Tsum Tau
1.Centrifuge nrog viav vias- thoob (nyiam dua) los yog lub kaum sab xis rotor nrog qhov dej / cov nqa khoom uas tuaj yeem ua tau 18 hli × 125mm 15 mL conical-bottomed hlab
CEEB TOOM: Kom tsis txhob muaj kev puas tsuaj rau lub cuab yeej thaum lub sij hawm centrifugation, xyuas kom meej meej ua ntej spinning.
2.Pipettor nrog 150 microliter (μL) cov lus qhia rau kev mloog zoo

3.Xaiv qhov tseeb MWCO
Once sample volume is determined, the next step is to select the appropriate MWCO (for ultrafiltration) or pore size (for microfiltration). MWCOs are nominal ratings based on the ability to retain >90 feem pua ​​​​ntawm cov ntsiab lus ntawm qhov hnyav molecular paub (hauv Kilodaltons). Lub rooj muab cov yam ntxwv tuav ntawm MWCO daim nyias nyias rau qee cov kuab tshuaj. Rau cov proteins, nws raug pom zoo kom xaiv MWCO uas yog peb mus rau rau lub sij hawm me dua li qhov hnyav molecular ntawm cov kua nplaum tau khaws cia. Yog tias tus nqi ntws yog ib qho kev txiav txim siab, xaiv daim nyias nyias nrog MWCO ntawm qhov qis kawg ntawm qhov ntau (3X); Yog tias qhov kev txhawj xeeb tseem ceeb yog khaws cia, xaiv ib daim nyias nyias (6X). Nws yog ib qho tseem ceeb kom paub tias qhov tuav ntawm ib lub molecule los ntawm ib qho ultrafiltration membrane yog txiav txim los ntawm ntau yam, ntawm cov uas nws qhov hnyav molecular ua hauj lwm tsuas yog raws li ib tug general qhia.
Yog li ntawd, xaiv qhov tsim nyog MWCO rau ib daim ntawv thov tshwj xeeb yuav tsum tau xav txog ntau yam xws li cov qauv molecular, tus nqi hluav taws xob, cov qauv concentration, qauv qauv, thiab kev ua haujlwm. Vim tias cov tuam txhab sib txawv siv cov khoom sib txawv los txheeb xyuas MWCO ntawm lawv cov daim nyias nyias, nws yog ib qho tseem ceeb uas yuav tsum tau ua cov kev sim ua kom pom tseeb ntawm daim nyias nyias hauv ib daim ntawv thov.

Common variables uas nce Molecule Passage:
1.Linear piv rau globular molecules.
2.High transmembrane siab tsim los ntawm g-force hauv centrifugal concentrators. (Qhov no yog ib qho tseem ceeb tshwj xeeb tshaj yog nyob rau hauv cov ntaub ntawv ntawm linear molecules, piv txwv li DNA tawg.
3.Buffer muaj pes tsawg leeg uas nyiam kev tawg ntawm molecules.
4.pH thiab ionic tej yam kev mob uas hloov cov molecule
5.Tsawg kub (5 degree piv rau 25 degree).
MWCO Kev Xaiv rau Cov Ntawv Thov Protein

4. Cov ntawv thov
Cov khoom siv centrifugal tuaj yeem hloov cov txheej txheem sib cais ib txwm muaj, xws li kab chromatography, kev npaj electrophoresies, cawv lossis ntsev los nag, lim ntshav, thiab gradient centrifugation, thaum ua cov hauv qab no:
Protein los yog nucleic acid concentration
Desalting, tsis pauv, lossis diafiltration
Deproteination ntawm cov tshuaj lom neeg
Fractionation ntawm cov protein sib tov
Kev sib cais ntawm primers los ntawm PCR cov khoom
Kev sib cais ntawm cov ntawv sau npe nucleic acids lossis
Tus kab mob concentration lossis tshem tawm
Kev nthuav qhia ntawm cell lysates thiab cov ntaub so ntswg homogenates

5.Ordering Information

Cim npe nrov: 5ml centrifugal li, Tuam Tshoj, lwm tus neeg, manufacturers, hoobkas, lag luam wholesale, bulk, Tshuag, dawb qauv