Cov ntaub ntawv technical thiab kev siv tangential flow filtration system
Txawm hais tias nyob rau hauv cov khoom noj, tshuaj lossis kev lag luam lom neeg, kev ua kom huv yog ib kauj ruam tseem ceeb ntsig txog cov khoom zoo. Muaj ntau txoj hauv kev ua kom huv huv, suav nrog chromatography, centrifugation, lim ntshav thiab lwm yam. Qhov tseeb, lwm lub tshuab lim dej yog ntsiag to tawm, uas yog tangential flow filtering (TFF).
Yog li, hom kev siv tshuab dab tsi yog tangential flow filtration? Nws tuaj yeem nkag siab los ntawm kev sim yooj yim. Piv txwv tias koj muaj ib pawg ntawm pebbles thiab xuab zeb uas koj xav tau cais. Tej zaum koj yuav hais, qhov ntawd tsis yooj yim. Cia li nrhiav ib tug sieve. Qhov tseeb, tej yam tsis yooj yim li kev xav.
Thaum cov xuab zeb me me poob los ntawm qhov screen, pebble txheej yuav raug tsim rau ntawm qhov screen kom tiv thaiv cov xuab zeb nyob rau sab saum toj los ntawm kev hla. Qhov no yog qhov kawg filtration. Lub sijhawm no, nco ntsoov koj kom co maj mam. Qhov tseeb, qhov no yog lub hauv paus ntsiab lus ntawm tangential flow filtration. Lub vibrating npo tso cov pebbles thaiv qhov screen, thiaj li ua kom cov xuab zeb nyob rau sab saum toj dhau sai.
Rau ib txwm pom, vim hais tias cov dej ntws ntawm cov kua yog perpendicular mus rau saum npoo ntawm lub lim membrane, txoj kev no tseem hu ua tuag kawg pom. Qhov ntxeev yog tangential flow filtering, tseem hu ua cross flow filtering. Raws li lub npe qhia, cov kev taw qhia ntws ntawm cov kua yog sib npaug mus rau qhov chaw ntawm lub lim membrane.
Vim hais tias cov tangential ntws tsis tu ncua "ntxuav" qhov chaw ntawm lub lim membrane thaum lub sij hawm pom cov txheej txheem, qhov kev ua hauj lwm hom no zoo alleviates lub tsub zuj zuj ntawm loj hais thiab molecules ntawm daim nyias nyias, yog li nws muaj peev xwm cais thiab purify biomolecules sai thiab zoo. Nws qhov zoo yog raws li hauv qab no: piv nrog rau kev lim ntshav, kev sib dhos yooj yim dua thiab kev ua haujlwm nrawm dua; Piv nrog rau centrifugal concentration ntaus ntawv los yog stirred ultrafiltration ntaus ntawv, siab concentration yuav tau nyob rau hauv ib tug luv luv lub sij hawm.
Cov thev naus laus zis no tau siv dav hauv biology, chaw muag tshuaj thiab kev lag luam zaub mov. Nws tuaj yeem siv rau qhov concentration thiab desalination ntawm cov protein, peptides lossis nucleic acids, kev sib cais ntawm biomolecules ntawm ntau qhov sib txawv, kev sau ntawm cell ncua, thiab kev qhia meej ntawm fermentation broth thiab cell lysate.
