FAQs txog Centrifugal Filter Devices
Q1. Nws yog ib qho tsim nyog los cais cov proteins ntawm qhov sib txawv ntawm qhov hnyav molecular, yog li qhov sib txawv ntawm qhov hnyav molecular ntawm cov protein xav tau ntau npaum li cas?
A: Qhov loj ntawm ob lub protein yuav tsum txawv ntawm 10 npaug lossis ntau dua.
Q2. Muaj peev xwm centrifugalalCov cuab yeej lim tau ua kom tsis muaj menyuam thiab tiv taus alkali ntxuav?
A: Nws tuaj yeem ua kom tsis muaj menyuam nrog 75 feem pua ethanol aqueous tov, thiab daim nyias nyias tuaj yeem tiv taus 0.1-0.5N sodium hydroxide alkali ntxuav.
Q3. Yuav ua li cas nrog yog tias cov protein precipitated thaum lub sij hawm concentration?
A: Yog hais tias cov protein yog concentrated sai heev los yog concentrated heev, nws yuav ua rau cov protein los nag, thiab qhov kawg concentration ntawm cov protein tom qab concentration yuav tsum tsis txhob siab dhau lawm; Proteins uas yog rhiab heev rau concentration npaum li cas thiab yuav ua rau precipitate, qhia txoj kev txhim kho:
Txo qhov ceev centrifugal
Siv ultrafiltration membrane nrog ntau dua molecular hnyav txiav tawm
Lub tshuab nqus tsev twj tso kua mis thiab centrifuge
Q4. Dab tsi yog qhov ua tau yog vim li cas lubcentrifugal lim cov khoom sivtsis tuaj yeem centrifuged?
A: Koj tuaj yeem siv li ntawm 20 feem pua ethanol aqueous tov rau thawj zaug centrifugation yog tias lub raj ultrafiltration tsis tuaj yeem centrifuged, vim tias cov ultrafiltration membrane yog qhuav tag, lub ntsej muag ntawm daim nyias nyias yog tsis muaj zog hydrophobic, thiab cov flux tuaj yeem rov qab los tom qab infiltration nrog qis. nto tension solvent.
Q5. Txoj kev siv kom raug thiab khaws cia cov khoom siv lim centrifugal
A: Cov khoom siv lim dej centrifugal yuav tsum tau centrifuged nrog dej huv ua ntej siv los xyuas nws txoj kev ncaj ncees. Tom qab txhua qhov kev siv, nws yuav tsum tau muab cia rau hauv lub xeev ntub dej kom tsis txhob muaj cov membrane qhuav.
Q6. Yuav ua li cas cov centrifugal lim cov cuab yeej tshem tawm endotoxin?
A: Cov khoom siv lim dej centrifugal peb muab tsis tau kho nrog endotoxins, thiab vim hais tias endotoxins muaj dav nyob rau hauv cov xwm txheej, cov qauv uas xav tau endotoxins tuaj yeem ntxuav ua ntej nrog 0.5N sodium hydroxide ua ntej kev sim, thiab tom qab ntawd ntxuav nrog RO dej kom tshem tawm feem ntau ntawm cov endotoxin.
Q7. Thaum cov concentrated protein yog siv rau downstream tsom xam, nws pom tias muaj kev cuam tshuam, yog vim li cas?
A: Cov khoom siv lim centrifugal tuaj yeem ntxuav ua ntej nrog cov tshuaj tsis haum lossis dej huv. Yog tias tseem muaj kev cuam tshuam, ntxuav nrog 0.1N sodium hydroxide, thiab tom qab ntawd ntxuav nrog dej huv.
Q8. It pom tau tias tsis muaj lub hom phiaj protein nyob rau hauv cov concentrate tom qab concentration. Yuav ua li cas yog vim li cas?
A: 1) Thawj qhov concentration ntawm cov protein yuav tsum tsis txhob qis dhau, yog tias qhov concentration tsawg dhau, daim nyias nyias yuav tsis tuaj yeem khaws cia.
2) Xaiv qhov tsim nyog membrane pore loj. Lub centrifugal concentration ntawm lim raj belongs rau tuag-kawg pom, thiab daim nyias nyias pore loj yog feem ntau tsawg tshaj li 2 zaug los yog ntau tshaj lub hom phiaj protein.
3) Xaiv txoj cai centrifugal ceev
4) Seb lub hom phiaj protein precipitates
Q9. Passivation kho ntawm centrifugal lim cov cuab yeej
A: Passivation pretreatment ntawm qhov chaw ntawm cov khoom siv lim dej centrifugal tuaj yeem txo qhov adsorption poob ntawm cov protein ntawm daim nyias nyias, feem ntau, pretreatment ntawm kem ua ntej concentrating cov diluted protein tov yuav txhim kho qhov rov qab, vim hais tias cov tshuaj muaj peev xwm sau cov exposed. Npliag protein adsorption qhov chaw ntawm daim nyias nyias thiab nto, txoj kev passivation yog pre-soak lub kem nrog ib tug siab ntim ntawm passivation tov rau ntau tshaj 1 teev, yaug lub kem kom huv si nrog dej distilled, thiab ces centrifugate lub kem ib zaug nrog dej distilled. kom tshem tawm tag nrho cov tshuaj passivation uas yuav nyob twj ywm rau ntawm daim nyias nyias, ceev faj kom tsis txhob cia lub membrane qhuav tom qab passivation, yog tias koj xav khaws cia rau yav tom ntej siv, koj yuav tsum tau ntxiv cov dej distilled tsis muaj dej kom cov membrane ntub., thov hu rau peb cov engineers rau kev daws teeb meem passivation.
Q10. Puas yog ultrafiltration membrane lim cov molecules hierarchically?
A: Generally speaking, if the dead-end filtration method is used, it is difficult to accurately remove small molecules among several large molecules by ultrafiltration method. This is because the membrane is a porous network structure, and its filtration mechanism is based on retention rather than passage, such as, the cut off rate of molecules larger than 100kd is >95 feem pua yog tias siv 100kd ultrafiltration membrane, txawm li cas los xij, cov molecules me dua qhov pore loj ntawm daim nyias nyias tsis dhau los ntawm daim nyias nyias. Lub molecular retention tus nqi ntawm 67kd yog ntau tshaj 70 feem pua, thiab cov protein ntawm 43kd kuj yog ntau tshaj li 40 feem pua raug txiav tawm, ntau yam Cheebtsam nyob rau hauv ib tug tov tov dhau los ntawm daim nyias nyias tsis yooj yim thiab yooj yim raws li ib qho kev daws teeb meem, Hauv kev xyaum , yog tias koj xav cais ob macromolecules, qhov sib txawv ntawm qhov hnyav ntawm lawv yuav tsum yog yim zaug lossis ntau dua.
Q11. Yuav ua li cas cais cov loj thiab me me molecules los ntawm ultrafiltration?
A: Vim tias qhov kev xav tau ntawm cov txheej txheem, cov impurities hauv macromolecules raug tshem tawm, lossis cov molecules me me raug tshem tawm ntawm macromolecules, nws yog qhov qub hauv kev tsim khoom. Nws yog ib qho tsim nyog los xaiv qhov tsim nyog membrane pore loj, pom txoj kev thiab kev ua haujlwm. Feem ntau hais lus, yog tias MWCO specification ntawm daim nyias nyias raug xaiv ntawm ob lub proteins, kev sib cais zoo tshaj plaws tuaj yeem ua tiav. Yog hais tias cov kua protein yog diluted, kev khiav hauj lwm nyob rau hauv qis siab kuj yuav pab kom ua tau zoo dua. Xaiv tangential flow filtration thiab ua ntau yam diafiltration kom tau txais kev sib cais zoo dua.
