Tej yam cuam tshuam rau kev tshem tawm cov kab mob
Tej yam cuam tshuam rau kev tshem tawm cov kab mob
Kev tshem tawm tus kab mob tuaj yeem tshem tawm cov kab mob loj hauv cov kev daws teeb meem, thiab nws cov txiaj ntsig tshem tawm muaj feem cuam tshuam nrog qhov chaw tshem tawm cov kab mob pom, cov protein ntau ntawm cov kua noj, pom kev siab, lub sijhawm cia thiab cryopreservation, thiab lwm yam.
1. Qhov chaw tshem tawm cov kab mob pom tshwm sim
Kev tshem tawm tus kab mob tuaj yeem tshwm sim nyob txhua qhov chaw hauv kev ua kom huv hauv qab, tab sis nws feem ntau tshwm sim tom qab pH tus kab mob tsis ua haujlwm, cov kauj ruam nruab nrab chromatography lossis cov kauj ruam kawg chromatography. Zuag qhia tag nrho, thoob plaws hauv cov txheej txheem qis qis, qhov yuav tsum tau ua kom pom tseeb yog nyob ntawm qhov chaw ntawm tus kab mob pom cov kauj ruam nyob, raws li cov protein ntau, purity thiab cov txheej txheem ntim tuaj yeem sib txawv.
2. Protein concentration ntawm cov kua pub
Cov protein concentration ntawm cov qauv yuav cuam tshuam cov flux ntawm tus kab mob tshem tawm pom los ntawm kev sib cuam tshuam ntawm cov tshuaj noj thiab lub hnab membrane. Ib qho protein ntau dua yuav txo tus nqi ntawm tus kab mob tshem tawm cov txheej txheem pom. Nws kuj muaj feem xyuam rau cov khoom ntawm cov protein nws tus kheej thiab daim nyias nyias. Feem ntau, cov khoom concentration yog txo thaum lub sij hawm tas li dilution ntawm cov khoom kom muaj peev xwm thiab txaus tus nqi ntawm tus kab mob tshem tawm lim membrane.
3. Lim siab
Cov txheej txheem ntawm kev tshem tawm cov kab mob pom feem ntau yuav tsum tau ua kom pom kev tsis tu ncua. Vim tias qhov kev cuam tshuam siab tuaj yeem ua rau tus kab mob nkag mus. Lub hauv paus ntsiab lus yog: cov nyhuv convective ntawm cov kua dej ntws los ntawm lub lim ua rau cov kab mob ua rau cov kab mob nkag mus rau ntawm daim nyias nyias los yog dai rau hauv qhov me me ntawm qhov pore vim yog kev cais tawm ntawm molecular loj. Thaum lub siab poob qis, tus nqi ntws thiab cov nyhuv convection txo. Tus kab mob no yuav khiav tawm thiab tso tawm ntawm qhov hws me me, thiab kis mus rau qhov hws loj nrog qhov muaj feem ntau nkag mus.
4. Lub sij hawm cia thiab cryopreservation
Qee cov proteins sib sau ua ke thaum lub sijhawm khaws cia, tshwj xeeb tshaj yog thaum khaws cia cia. Cov kev sib sau no tuaj yeem cuam tshuam rau kev tshem tawm cov kab mob. Yog li ntawd, nws yuav tsum tau soj ntsuam xyuas yog tias muaj lub sijhawm ntev ntawm kev khaws cia hauv cov txheej txheem tsim khoom lossis thaum twg yog qhov yuav tsum tau cryopreservation.
