Cryopreservation - Ua kom cov cell viability thaum khov cell kab lis kev cai

Kev tswj kom tiav yog qhov tseem ceeb thaum koj ua haujlwm nrog cov kab lis kev cai ntawm tes. Cov kab lis kev cai ntawm tes siv tau yog qhov muaj txiaj ntsig zoo thiab khov lawv tuaj yeem ua kom lawv tau khaws cia rau yav tom ntej. Txawm li cas los xij, txoj haujlwm ntawm cov kab lis kev cai khov ntawm tes (cryopreservation) tuaj yeem tuaj nrog nws cov teeb meem nyuaj.

Cia peb saib ze dua ntawm cov teeb meem no thiab peb tuaj yeem kov yeej lawv li cas, kom ntseeg tau tias cell viability thaum lub sij hawm cryopreservation.

Dab tsi yog cov ntsiab lus tseem ceeb rau cryopreservation?

Thaum lub sij hawm cryopreservation, cov ntaub ntawv thiab cov khoom siv uas koj siv yog tus yuam sij rau kev vam meej ntawm khov lub hlwb kom zoo. Tsis yog tsuas yog ua kom muaj kev ncaj ncees ntawm cov txheej txheem tab sis kuj tseem cuam tshuam cov nqi thiab lub sijhawm.

Txoj hauv kev zoo rau kev khaws cia ntawm tes kab lis kev cai yog siv cov kua nitrogen, tab sis ua haujlwm ntawm cov kua nitrogen kub (-196 degree) tuaj yeem ua rau muaj teeb meem. Yog tias koj lub hlwb khov sai heev nws tuaj yeem ua rau cov dej khov ua kom muaj zog, ua rau cov membrane puas thiab cell tuag. Kev siv cov tshuaj tiv thaiv cryoprotective, xws li dimethyl sulfoxide (DMSO) feem ntau yog siv los txo qhov chaw khov thiab tso cai rau qhov txias txias.

Nrhiav lub thawv khaws cia uas tiv thaiv koj lub hlwb

Nrog rau cov kev pheej hmoo hauv siab, nws yog ib qho tseem ceeb kom nrhiav tau ib qho kev daws teeb meem uas yog ob qho tib si tshuaj lom neeg thiab hloov tau ntawm qhov kub thiab txias siv thaum lub sij hawm cryopreservation.

Ib leeg-siv bioprocess hnab yog ib qho kev daws teeb meem universally siv nyob rau hauv feem ntau bio-applications. Lawv muab zoo biocompatibility, tshuaj compatibility, barrier thiab zog zog. Nrog lub peev xwm los hloov kho kom haum rau cov kev xav tau tshwj xeeb ntawm koj cov txheej txheem, muab kev yooj yim txij thaum pib mus txog thaum xaus.

Peb puas tuaj yeem siv qhov kev ntseeg siab no kom ua tau raws li qhov xav tau ntawm cryopreservation?