FAQs txog Microfiltration & Ultrafiltration Cassettes
FAQs txog Microfiltration & Ultrafiltration Cassettes
Q1. Yuav ua li cas xaiv qhov tsim nyog transmembrane siab?
A: Transmembrane siab muaj feem xyuam rau tus nqi pub, pub kua viscosity, membrane pore loj, thiab diaphragm valve hloov. Feem ntau, lub siab transmembrane tswj ntawm {{0}} bar; me me 0.11 square meter membrane packs yog siv nyob rau hauv ib thooj thiab feem ntau yog siv rau kev tshawb fawb thiab kev loj hlob. Lub siab transmembrane feem ntau yog kho los ntawm me me mus rau siab, thiab qhov ntau yog tswj ntawm 0-2bar. Yog tias nws yog thawj zaug ua tangential ntws, koj tuaj yeem teeb tsa lub siab pub rau 1 bar thiab rov qab siab txog li 0.5 bar rau kev sim.
Q2. Yuav ua li cas xaiv tus khiav tus nqi ntawm peristaltic twj tso kua mis?
A: Tus nqi ntws ntawm lub twj tso kua mis dav dav yog teem raws li thaj tsam membrane. Qhov pom zoo pub tus nqi ib square meter ntawm thaj chaw membrane rau ultrafiltration cassettes yog 200-400L / h; qhov pom zoo pub tus nqi ib square meter ntawm thaj chaw membrane rau microfiltration cassettes yog 400-600L / h; Tsis tas li ntawd, qhov me me ntawm daim nyias nyias pore loj, qhov qis ntawm cov khoom noj feem ntau, vim tias qhov siab ntawm transmembrane yuav nce siab ntawm cov nqi ntws.
Q3. Yuav ua li cas ntxuav lub cassette thiab cov txheej txheem ntxuav yog dab tsi?
A: Rau ultrafiltration thiab microfiltration cassettes, tom qab ntxuav nrog dej, ntxuav nrog {{{0}}.5M NaOH rau 30 feeb, thiab ces nkag mus rau lye nrog lub diaphragm valve qhib tag nrho. Tom qab reflux pH nce, cov yeeb nkab xa rov qab muab tso rau hauv lub khob lye. Lub diaphragm valve rotates kom lub reflux siab ntsuas mus txog 0.2bar. Peb lub raj yog ntxuav hauv ib lub thawv rau 30 feeb; ces lawv tau khaws cia nrog 0.1M NaOH. Tom qab ntxuav lub 1kd thiab 3kd ultrafiltration membrane nrog lye, nws yuav tsum tau ntxuav nrog dej kom nruab nrab thiab ces muab cia rau hauv 1.5 feem pua sodium bisulfite.
Kev tu tus txheej txheem: Lub membrane flux rov qab mus rau ntau tshaj 90 feem pua ntawm cov thawj membrane flux.
Q4.Yuav ua li cas kuaj dej flux?
A: Txhawm rau xaiv tus nqi pub, kho lub siab transmembrane ntawm lub diaphragm valve rau 1.5bar thiab ntsuas LMH tus nqi ntawm cov dej hauv qhov permeate kawg. Tom qab siv cov membrane cassette txhua zaus, yog tias koj yuav tsum tau soj ntsuam lub neej ntawm cov membrane pob los yog seb nws puas tau ntxuav, koj tuaj yeem khaws tib tus nqi pub thiab transmembrane siab raws li saum toj no rau kev kuaj.
Q5. Yuav ua li cas xaiv lub membrane pore loj?
A: Rau ultrafiltration concentration: koj tuaj yeem xaiv lub membrane pore loj uas yog {{0}} zaus me dua li cov hom phiaj protein. Piv txwv li, yog tias lub hom phiaj protein yog 150kd, koj tuaj yeem xaiv 30kd lossis 50kd membrane cassette. Kom paub meej thiab pom: supernatant tom qab crushing poov xab, CHO cell kab lis kev cai nruab nrab, thiab lwm yam., feem ntau xaiv 0.22 los yog 0.45μm membrane cassette.
Q6.Yuav ua li cas xaiv qhov chaw membrane?
A: Raws li cov qauv ua ntim thiab cov neeg siv khoom xav tau lub sijhawm ua haujlwm los xaiv qhov tsim nyog ntawm daim nyias nyias. Feem ntau, qhov pom zoo kho ntim ntawm 0.11m2 membrane cassette yog {{0}}.5-4L, qhov pom zoo kho ntim ntawm 0.5m2membrane cassette yog 4-20L, thiab pom zoo kho ntim ntawm 1.3m2 membrane cassette yog 20-100L. Piv txwv li, cov qauv ntim ntawm cov neeg siv khoom yog 150L, thiab qhov hnyav molecular ntawm lub hom phiaj protein yog 30kd. Nws yuav tsum ua kom tiav qhov concentration ntawm 150L qauv mus rau 10L hauv 2 teev. Lub LMH tau txais los ntawm kev siv 5kd membrane cassette nyob rau hauv ib qho me me ntawm kev sim yog 20. Qhov chaw ntawm daim nyias nyias tuaj yeem suav tau raws li qhov muaj peev xwm kho tau thiab LMH. 140L/20LMH/2h{11}}.5m2.
Q7. Dab tsi yog lifespan thiab siv sij hawm ntawm ultrafiltration membranecassette?
A: Lub neej kev pab cuam ntawm lub hnab membrane yog ntev heev. Nyob rau hauv qhov chaw ntawm kev siv ib txwm thiab kev saib xyuas, lub neej ntev thiab kev siv lub sij hawm ntawm daim nyias nyias nyias tuaj yeem ntev dua qhov ntim. Ua kom rov tsim dua tshiab, zam kev sib cuag nrog qee qhov tsis paub txog cov organic reagents thiab ua kom lawv tsim nyog.
Q8. Dab tsi yog qhov txawv ntawm tangential flow velocity thiab membrane flux?
A: Membrane flux yog hais txog cov membrane permeation flux. Kev nce hauv tangential txaus tus nqi yuav pab txhawb qhov scouring ntawm macromolecules ntawm daim nyias nyias, thiab permeation tus nqi ntawm me me molecules yuav nce. Feem ntau, qhov ntau dua qhov tangential ntws tus nqi, qhov ntau dua cov membrane flux.
q9 ua. Puas tuaj yeem siv cov khoom ntsuas kev ncaj ncees los kuaj ntau daim kab xev?
A: Tus neeg ntsuas kev ncaj ncees yuav muaj qee qhov kev txwv, tus neeg ntsuas kev ncaj ncees feem ntau muaj qhov txwv txog li 1000mL / min, yog tias ntsuas tus nqi ntawm daim nyias nyias nyias ntau dua qhov kev txwv no, tus ntsuas kev ncaj ncees tsis haum rau kev sim. Nws raug nquahu kom koj siv barometer nrog tus nqi ntws loj dua txhawm rau txheeb xyuas.
Q10. Thaum txhim kho lub membrane cassette, nws tau hais tias"xyuas seb lub torque puas nyob hauv qhov chaw", yuav ua li cas xyuas lub torque tshwj xeeb?
A: Thaum txhim kho, ua ntej ntawm tag nrho cov, yog hais tias lub torque tau mus txog, yuav muaj ib tug tawm tswv yim, thiab lub torque ciaj ntswj yuav ceeb toom rau koj tias nws tau los txog. Yog tias muaj qhov hloov pauv ntawm qhov kub thiab txias, xws li thermal expansion thiab contraction, nws raug nquahu kom koj nce lub zog kom tsim nyog thaum nws txias, thiab kho nws dua. Yog hais tias cov tshuaj yog kub, nws raug nquahu kom loosen lub clamp ua ntej ntxiv cov tshuaj tiv thaiv kom tsis txhob muaj cov membrane cassette cuam tshuam los ntawm thermal expansion.
Q11. Thaum lub sij hawm ultrafiltration, thaum cov tshuaj tsis txaus, yuav tsum kaw qhov permeate kawg, voj voog tos lossis nres tos?
A: Yog tias qhov kev daws teeb meem tsis txaus, koj yuav tsum kaw qhov permeate kawg thiab lub voj voog tos. Nws tsis pom zoo kom nres tos, vim tias yog tias koj qhov kev daws teeb meem nres ntawm qhov chaw ntawm daim nyias nyias, nws yuav raug hloov pauv qee yam khoom thiab cuam tshuam rau kev pom. Nws yog feem ntau pom zoo kom yaug cov khoom hauv lub sijhawm thiab tso cov khoom tawm nrog lub taub hau cua.
Q12. Puas yuav muaj kev cuam tshuam rau kev tso tseg lub twj tso kua mis thaum lub sij hawm concentration lossis diafiltration?
A: Thaum lub twj tso kua mis nres, yuav muaj qee yam khoom hloov pauv ntawm qhov chaw ntawm daim nyias nyias, uas yuav cuam tshuam cov txheej txheem ntawm koj cov permeation tom ntej.
q13 ua. Puas yog lub gasket ntawm daim nyias nyias nyias pov tseg lossis rov siv tau? Puas muaj kev tu kom huv?
A: Lub gasket ntawm daim nyias nyias tuaj yeem rov qab siv tau, tab sis nws raug pom zoo kom hloov nws txhua rau lub hlis thaum nws rov qab siv dua, vim tias lub gasket yuav deformed los yog laus thaum siv.
q14 ua. Lub concentration txaus tus nqi ntawm cov membrane cassette yog obviously slowed down. Koj puas muaj cov lus qhia txog kev tu kom zoo?
A: Thaum lub sij hawm ultrafiltration concentration txheej txheem ntawm membrane cassette, tus nqi txaus ntawm permeation kawg yuav maj mam txo. Vim yog qhov tsim ntawm lim ncuav mog qab zib txheej, cov flux yuav ua rau qis qis. Yog li ntawd, thaum tsim, lub peev xwm ua rau ib chav tsev ntim ntawm kev daws yog txiav txim siab. Nyob rau hauv qhov tseeb hais tias qhov permeation kawg txaus siab rau cov txheej txheem efficiency, xaiv qhov tsim nyog membrane cheeb tsam kom tsis txhob muaj qhov teeb meem ntawm qeeb qeeb. Feem ntau, los ntawm kev ua kom cov cheeb tsam membrane, cov txheej txheem yuav tsum tau ua tau zoo.
q15 ua. Yuav ua li cas txiav txim siab tias lub neej kev pab cuam ntawm lub membrane cassette tau tas sij hawm thiab siv tsis tau dua?
A: Qhov kev txiav txim siab no yog thawj zaug raws li lub sijhawm siv tau ntawm cov membrane cassette nws tus kheej, tom qab ntawd rov qab los ntawm cov dej ntws ntawm cov membrane cassette, uas yog ib qho xwm txheej rau kev saib xyuas seb qhov kev ua tau zoo ntawm cov membrane cassette rov qab los. Yuav muaj kev piav qhia pov thawj thaum ua cov txheej txheem membrane cassette. Qee zaum yuav muaj dej ntws tawm. Yog tias qhov zoo ntawm daim nyias nyias nyias thiab lwm qhov ntsuas tsis cuam tshuam, nws tuaj yeem siv tau raws li kev pabcuam lub neej ntawm cov membrane cassette. Qhov no xav kom peb tshawb nrhiav raws li cov txheej txheem tshwj xeeb. Feem ntau, tom qab 100 zaus ntawm kev siv lossis txog 2 xyoos, cov theem flux yuav raug txheeb xyuas los txiav txim seb nws puas yuav tsum tau hloov.
Q16. Yuav ua li cas txheeb xyuas cov nyhuv tu? Yuav ua li cas txheeb xyuas qhov degree ntawm kev tu kom tsim nyog?
A: Cov nyhuv tu yuav tsum kuaj TOC, endotoxin, microbial txwv, cov khoom seem, PH conductivity, thiab lwm yam tom qab ntxuav nrog dej. Qhov no suav nrog kev txheeb xyuas kev ntxuav, thiab kev npaj tu zaum kawg yuav tsum tau lees paub tom qab dhau qhov kev xeem no.
